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71.
Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome 总被引:1,自引:0,他引:1
An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified
and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing
four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.
Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309) 相似文献
72.
列出了不重视推断解释可靠性问题而导致的严重后果;论述了复杂情况下重、磁异常解释的思路和对策,即如何正确、合理使用方法技术;提出了推断成果可靠性划分方案的建议和新的成果表达方式的建议. 相似文献
73.
Introduction Based on the elastic theory of hard inclusion model proposed by Dobrovolskii (1991), we developed a rheologic inclusion model to study the spatial-temporal variation of earthquake pre-cursor by using the bulk-strain field resulted from rheologic inclusion model (SONG et al, 2000). Based on the elastic inclusion theory, the analytical expressions for the viscoelastic displacement field and strain field of rheologic inclusion model are derived (SONG et al, 2003, 2004). Further-m… 相似文献
74.
75.
使用软件1,将岩层序号、导线的方位和倾角、岩层的倾向、倾角和两层面切割导线处的读数分别输入A、F、G、H、I、J、K列,先后分别沿M、N列从第2行单元格开始拖动鼠标,岩层真厚度数据和累积厚度即刻计算出来,进一步可计岩组或岩性段的真厚度;将导线的序号、方位、倾角、长度分别输入W、X、Y、Z列,先后分别沿AB、AC、AD、AE、AF、AG、AH列,从第2行单元格开始拖动鼠标,导线的水平投影长度、纵座标增量及其累积值、横座标增量及其累积值、高程增量及其累积值即刻计算出来。使用软件2,在输入原始数据的同时,结果便计算出来。 相似文献
76.
Introduction The rock medium shows mainly elastic character under low-temperature, low-pressure and external force for short duration, yet it shows rheologic property under high-temperature, high-pressure and long-time external force. It is reasonable that the rapid earthquake generating process lasted for tens seconds is analyzed by the elastic theory. As for the earthquake generating process for hundreds or thousand years, nevertheless, the rheologic property of rock must be taken into accou… 相似文献
77.
谢圣婷 《广东海洋大学学报》2006,26(5):84-87
柳亚子是辛亥革命前后诗坛上影响最大的革命诗人之一.他在辛亥革命前后创作的大量诗词,追求理想,憧憬未来;关注现实,"爱"恨"交织;并以慷慨激昂,苍凉沉郁调子组成一曲曲激荡着宏音巨响的时代之歌,在中国近代的茫茫夜空中激荡震响,这些裹夹着雷霆风云与宏音巨响的诗歌创作在我国民主革命史和近代文学史上产生了重要影响,并放射出璀璨的光辉. 相似文献
78.
Davis JA May MD Greenfield BK Fairey R Roberts C Ichikawa G Stoelting MS Becker JS Tjeerdema RS 《Marine pollution bulletin》2002,44(10):1117-1129
In 1997, seven sport fish species were sampled from seven popular fishing areas in San Francisco Bay. Mercury exceeded a human health screening value in 44 of 84 (52%) samples. All collected samples of leopard shark and striped bass exceeded the mercury screening value of 0.23 microg/g wet weight. PCBs exceeded the screening value in 51 of 72 (71%) samples. DDT, chlordane, and dieldrin, had lower numbers of samples above screening values: 16 of 72 (22%) for DDT, 11 of 72 (15%) for chlordanes, and 27 of 72 (37%) for dieldrin. Concentrations of PCBs and other trace organics were highest in white croaker and shiner surfperch, the two species with the highest fat content in their muscle tissue. Fish from one location, Oakland Harbor, had significantly elevated wet weight concentrations of mercury, PCBs, DDTs, and chlordanes compared to other locations. Removal of skin from white croaker fillets reduced lipid concentrations by 27-49% and concentrations of trace organics by 33-40%. 相似文献
79.
The directionality of thermal radiance of a homogeneous isothermal non-black plane surface is totally decided by its directional emissivity, which depends on the complex dielectric constant and roughness of surface. It can be expressed by This paper proves that it is necessary to express emissivity by a matrix when a target becomes an inhomogeneous non-isothermal open complex with complicated inner geometric structure. The matrix describes the inner radiative interaction among components accurately and also expresses its thermal radiative directionality and structural characteristics completely. Advantages of matrix expression are as follows: first, the physical mechanics of effective emissivity of an open complex is described in a simple and perfect way; second, it becomes easy to understand the principle and method to retrieve components temperature from multi-angle thermal remotely sensed data; and third, the differences of directionalities between an open complex and a homogeneous isothermal non-black plane body are expressed by just using an effective emissivity matrix instead of an emissivity vector. Formula in classic physics is only the special case of matrix expression; therefore, the matrix is a universal unconditional expression to describe the directionality of thermal radiance. 相似文献
80.
土水特征曲线的通用数学模型研究 总被引:13,自引:3,他引:13
土水特征曲线对于研究非饱和土的物理力学特性至关重要。根据土水特征曲线可以确定非饱和土的强度、体应变和渗透系数 ,甚至可以确定地下水面以上水份分布。由于土体物理力学特性的差异 ,导致描述其土水特征曲线的数学模型也各不相同。因此 ,建立土水特征曲线的通用数学表达式 ,显得尤为必要。本文对土水特征曲线数学模型进行了研究 ,依据这些模型的数学表达式形式 ,将其划分为 4种类型。分别由这 4种类型的数学模型推导出具有统一表达式形式的土水特征曲线通用数学模型 ,并运用陕北高原黄土土水特征曲线试验数据对通用数学模型进行了研究 相似文献